Coding

Part:BBa_K3515011:Experience

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-29)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3515011

User Reviews

UNIQbce10734a21395c5-partinfo-00000000-QINU UNIQbce10734a21395c5-partinfo-00000001-QINU

Improvements

Northern_BC iGEM 2021 Vitamin D Biosensor:


In 2021, team Northern_BC adapted part BBa_K3515011 for its use in a vitamin D biosensor. Improvements of this part included the removal of a stop codon in the middle of the sequence, codon optimization for expression in an E. coli system, the addition of a C-terminal His-tag, and the removal of restriction sites to ensure RFC[10, 12, 23, 25, and 100] BioBrick compatibilities.

The improved part containing these edits is BBa_K3957000.